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hhnscc cell lines ut-scc-15  (SAS institute)


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    SAS institute hhnscc cell lines ut-scc-15
    Hhnscc Cell Lines Ut Scc 15, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ut-scc-15/hhnscc+cell+lines+ut+scc+15/pm21641070-1-9-14
    Average 90 stars, based on 1 article reviews
    hhnscc cell lines ut-scc-15 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Changes in the fraction of total hypoxia and hypoxia subtypes in human squamous cell carcinomas upon fractionated irradiation: evaluation using pattern recognition in microcirculatory supply units.
    Article Snippet: Background and purpose: Evaluate changes in total hypoxia and hypoxia subtypes in vital tumor tissue of human head and neck squamous cell carcinomas (hHNSCC) upon fractionated irradiation.. Materials and methods: Xenograft tumors were generated from 5 hHNSCC cell lines (UT-SCC-15, FaDu, SAS, UT-SCC-5 and UT-SCC-14).. Hypoxia subtypes were quantified in cryosections based on (immuno-)fluorescent marker distribution patterns of Hoechst 33342 (perfusion), pimonidazole (hypoxia) and CD31 (endothelium) in microcirculatory supply units (MCSUs).



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    Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYNU, KYAT2, KYAT3, and KYAT4 in glioblastoma multiforme <t>(GBM)</t> and head and neck squamous cell carcinoma cells (HNSCC). The graphs indicate the mRNA expression normalized to the housekeeping genes (2 −Δ CT). GBM ( N = <t>13,</t> <t>HROG02,</t> <t>HROG04,</t> <t>HROG05,</t> <t>HROG06,</t> <t>HROG10,</t> <t>HROG15,</t> <t>HROG24,</t> <t>HROG36,</t> <t>HROG38,</t> <t>HROG52,</t> <t>HROG63,</t> <t>HROG73,</t> <t>HROG75)</t> and HNSCC cell lines [ N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC14, UT-SCC-15 ].
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    Image Search Results


    Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYNU, KYAT2, KYAT3, and KYAT4 in glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma cells (HNSCC). The graphs indicate the mRNA expression normalized to the housekeeping genes (2 −Δ CT). GBM ( N = 13, HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines [ N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC14, UT-SCC-15 ].

    Journal: Frontiers in Immunology

    Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

    doi: 10.3389/fimmu.2020.00055

    Figure Lengend Snippet: Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYNU, KYAT2, KYAT3, and KYAT4 in glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma cells (HNSCC). The graphs indicate the mRNA expression normalized to the housekeeping genes (2 −Δ CT). GBM ( N = 13, HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines [ N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC14, UT-SCC-15 ].

    Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

    Techniques: Expressing

    Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYNU, KYAT2, KYAT3 , and KYAT4 as well as IDO1 protein abundance following interferon-γ (IFNγ) stimulation in glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma (HNSCC) cells. The cell cultures were either untreated or treated with IFNγ (50 ng/ml) for 24 h. (A) The graphs indicate the mRNA expression normalized to the housekeeping genes (2 −Δ CT). GBM N = 5 cell lines (HROG02, HROG05, HROG52, HROG63, HROG75); HNSCC, N = 4 cell lines (FADU, Detroit-562, Cal-33, PE/CA/PJ-15). (B) IDO1 immunofluorescence in selected cell lines. Cell nuclei were stained with DAPI, and IDO1 was depicted by monoclonal rabbit IDO1 primary antibody (1:100; Cell Signaling Technology), followed by secondary antibody (1:250, Boster Biological Technology Pleasanton, CA, United States) labeling. Cells were analyzed with a Zeiss LSM-780 Confocal Laser Microscope. Original magnification 20×.

    Journal: Frontiers in Immunology

    Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

    doi: 10.3389/fimmu.2020.00055

    Figure Lengend Snippet: Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYNU, KYAT2, KYAT3 , and KYAT4 as well as IDO1 protein abundance following interferon-γ (IFNγ) stimulation in glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma (HNSCC) cells. The cell cultures were either untreated or treated with IFNγ (50 ng/ml) for 24 h. (A) The graphs indicate the mRNA expression normalized to the housekeeping genes (2 −Δ CT). GBM N = 5 cell lines (HROG02, HROG05, HROG52, HROG63, HROG75); HNSCC, N = 4 cell lines (FADU, Detroit-562, Cal-33, PE/CA/PJ-15). (B) IDO1 immunofluorescence in selected cell lines. Cell nuclei were stained with DAPI, and IDO1 was depicted by monoclonal rabbit IDO1 primary antibody (1:100; Cell Signaling Technology), followed by secondary antibody (1:250, Boster Biological Technology Pleasanton, CA, United States) labeling. Cells were analyzed with a Zeiss LSM-780 Confocal Laser Microscope. Original magnification 20×.

    Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

    Techniques: Expressing, Quantitative Proteomics, Immunofluorescence, Staining, Labeling, Microscopy

    Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYAT1, KYAT2, KYAT3 , and KYAT4 upon concomitant treatment with interferon-γ (IFNγ) and cytostatic drugs in glioblastoma multiforme (GBM) cells. The cell cultures ( N = 5, HROG02, HROG05, HROG52, HROG63, HROG75) were either left untreated or treated with IFNγ (50 ng/ml) for 24 h. Treatments were performed simultaneously, i.e., IFNγ ± TMZ, and/or Dinaciclib. Graphs indicate the mRNA expression of selected KP-related genes normalized to the housekeeping genes (2 −Δ CT). Results show data of three independent experiments. A statistical report is given in .

    Journal: Frontiers in Immunology

    Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

    doi: 10.3389/fimmu.2020.00055

    Figure Lengend Snippet: Relative messenger RNA (mRNA) expression of IDO1, TDO2, KYAT1, KYAT2, KYAT3 , and KYAT4 upon concomitant treatment with interferon-γ (IFNγ) and cytostatic drugs in glioblastoma multiforme (GBM) cells. The cell cultures ( N = 5, HROG02, HROG05, HROG52, HROG63, HROG75) were either left untreated or treated with IFNγ (50 ng/ml) for 24 h. Treatments were performed simultaneously, i.e., IFNγ ± TMZ, and/or Dinaciclib. Graphs indicate the mRNA expression of selected KP-related genes normalized to the housekeeping genes (2 −Δ CT). Results show data of three independent experiments. A statistical report is given in .

    Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

    Techniques: Expressing

    Indoleamine 2,3-dioxygenase (IDO1) protein abundance in selected glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma (HNSCC) cells upon cytostatic drugs and targeted therapy. The cell cultures [ (A) GBM: HROG05; (B) HNSCC: Detroit-562] were either left untreated or treated with IFNγ (50 ng/ml) for 24 h. Treatments were performed simultaneously, i.e., IFNγ ± TMZ, Cetuximab, and/or Dinaciclib. Cell nuclei were stained with DAPI. Original magnification 20×. (C,D) Quantification was done to score staining intensity in untreated and treated HROG05 and Detroit-562 cells. This was carried out using ImageJ software as described in Material and Methods.

    Journal: Frontiers in Immunology

    Article Title: Activation of the Kynurenine Pathway in Human Malignancies Can Be Suppressed by the Cyclin-Dependent Kinase Inhibitor Dinaciclib

    doi: 10.3389/fimmu.2020.00055

    Figure Lengend Snippet: Indoleamine 2,3-dioxygenase (IDO1) protein abundance in selected glioblastoma multiforme (GBM) and head and neck squamous cell carcinoma (HNSCC) cells upon cytostatic drugs and targeted therapy. The cell cultures [ (A) GBM: HROG05; (B) HNSCC: Detroit-562] were either left untreated or treated with IFNγ (50 ng/ml) for 24 h. Treatments were performed simultaneously, i.e., IFNγ ± TMZ, Cetuximab, and/or Dinaciclib. Cell nuclei were stained with DAPI. Original magnification 20×. (C,D) Quantification was done to score staining intensity in untreated and treated HROG05 and Detroit-562 cells. This was carried out using ImageJ software as described in Material and Methods.

    Article Snippet: Patient-derived GBM cell lines ( N = 13; HROG02, HROG04, HROG05, HROG06, HROG10, HROG15, HROG24, HROG36, HROG38, HROG52, HROG63, HROG73, HROG75) and HNSCC cell lines ( N = 6; FADU, Detroit-562, Cal-33, PE/CA/PJ-15, UT-SCC-14, UT-SCC-15) were either established and basically characterized in our lab or originally obtained from the German collection of cell cultures (DSMZ; Braunschweig, Germany).

    Techniques: Quantitative Proteomics, Staining, Software